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Estimation of intra-operator variability in perfusion parameter measurements using DCE-US

Abstract : AIM: To investigate intra-operator variability of semi- quantitative perfusion parameters using dynamic con- trast-enhanced ultrasonography (DCE-US), following bolus injections of SonoVue®. METHODS: The in vitro experiments were conducted using three in-house sets up based on pumping a fluid through a phantom placed in a water tank. In the in vivo experiments, B16F10 melanoma cells were xenografted to five nude mice. Both in vitro and in vivo , images were acquired following bolus injections of the ultra- sound contrast agent SonoVue® (Bracco, Milan, Italy) and using a Toshiba Aplio® ultrasound scanner connect- ed to a 2.9-5.8 MHz linear transducer (PZT, PLT 604AT probe) (Toshiba, Japan) allowing harmonic imaging (“Vascular Recognition Imaging”) involving linear raw data. A mathematical model based on the dye-dilution theory was developed by the Gustave Roussy Institute, Villejuif, France and used to evaluate seven perfusion parameters from time-intensity curves. Intra-operator variability analyses were based on determining perfu- sion parameter coefficients of variation (CV). RESULTS: In vitro, different volumes of SonoVue® were tested with the three phantoms: intra-operator variability was found to range from 2.33% to 23.72%. In vivo, experiments were performed on tumor tissues and perfusion parameters exhibited values ranging from 1.48% to 29.97%. In addition, the area under the curve (AUC) and the area under the wash-out (AUWO) were two of the parameters of great interest since throughout in vitro and in vivo experiments their vari- ability was lower than 15.79%. CONCLUSION: AUC and AUWO appear to be the most reliable parameters for assessing tumor perfusion using DCE-US as they exhibited the lowest CV values.
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Submitted on : Thursday, July 8, 2021 - 8:40:20 AM
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Marianne Gauthier, Ingrid Leguerney, Jessie Thalmensi, Mohamed Chebil, Sarah Parisot, et al.. Estimation of intra-operator variability in perfusion parameter measurements using DCE-US. World Journal of Radiology, Baishideng Publishing Group, 2011, 3, ⟨10.4329/wjr.v3.i3.70⟩. ⟨hal-03281153⟩



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