Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia

Abstract : Novel hyper-resistant bacteria were isolated from the Crven Dol mine (Allchar, North Macedonia), arsenic-rich extreme environment. Bacteria were recovered from a secondary mineral mixture, an alteration of hydrothermal realgar rich in arsenates (pharmacolite, hornesite, and talmessite). The sample was recovered from the dark part of the mine at 28 m depth. Three bacterial strains and a bacterial consortium were isolated for their capacity to survive exposure to 32 g/L (209 mM) of arsenite, and 176 g/L (564 mM) of arsenate. The 16S rRNA gene analysis identified bacterial isolates as Stenotrophomonas sp. and two Microbacterium spp. This analysis also revealed that bacterial consortium comprise two Bacteriodetes exhibiting similarity to Olivibacter ginsengisoli and to uncultured bacterium, and one γ-proteobacteria with similarity to Luteimonas sp. Among all isolates Stenotrophomonas sp. exhibited the highest tolerance to As compound as well as the capacity to accumulate As inside the cells. Analysis of genes involved in As-resistance showed that recovered isolates possess the genes encoding the ArsB, Acr3(1) and Acr3(2) proteins, indicating that at least a part of their resistance could be ascribed to As-efflux systems described in isolates obtained from human-polluted environments.
Liste complète des métadonnées

Littérature citée [44 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-02920366
Contributeur : Sylvie Blanc <>
Soumis le : jeudi 27 août 2020 - 18:10:36
Dernière modification le : vendredi 11 septembre 2020 - 14:32:27

Fichier

bermanec2020.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Vladimir Bermanec, Tina Paradžik, Snježana Kazazić, Chantelle Venter, Jasna Hrenović, et al.. Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia. Journal of Hazardous Materials, Elsevier, 2021, 402, pp.123437. ⟨10.1016/j.jhazmat.2020.123437⟩. ⟨hal-02920366⟩

Partager

Métriques

Consultations de la notice

122

Téléchargements de fichiers

54