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Mannose-centered aromatic galactoclusters inhibit the biofilm formation of Pseudomonas aeruginosa

Caroline Ligeour 1 Olivier Vidal 2 Lucie Dupin 3 Francesca Casoni 1 Emilie Gillon 4 Albert Meyer 1 Sébastien Vidal 5 Gérard Vergoten 2 Jean-Marie Lacroix 2 Eliane Souteyrand 3 Anne Imberty 4 Jean-Jacques Vasseur 1 Yann Chevolot 3 François Morvan 1
1 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
2 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576
3 INL - C&N - INL - Chimie et Nanobiotechnologies
INL - Institut des Nanotechnologies de Lyon
4 CERMAV - Centre de Recherches sur les Macromolécules Végétales
5 CO2GLYCO - Chimie Organique 2-Glycochimie
ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
Abstract : Pseudomonas aeruginosa (PA) is a major public health care issue due to its ability to develop antibiotic resistance mainly through adhesion and biofilm formation. Therefore, targeting the bacterial molecular arsenal involved in its adhesion and the formation of its biofilm appears as a promising tool against this pathogen. The galactose-binding LecA (or PA-IL) has been described as one of the PA virulence factors involved in these processes. Herein, the affinity of three tetravalent mannose-centered galactoclusters toward LecA was evaluated with five different bioanalytical methods: HIA, ELLA, SPR, ITC and DNA-based glycoarray. Inhibitory potential towards biofilms was then assessed for the two glycoclusters with highest affinity towards LecA (Kd values of 157 and 194 nM from ITC measurements). An inhibition of biofilm formation of 40% was found for these galactoclusters at 10 μM concentration. Applications of these macromolecules in anti-bacterial therapy are therefore possible through an anti-adhesive strategy.
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https://hal.archives-ouvertes.fr/hal-01317684
Contributor : Frédérique Depierre <>
Submitted on : Monday, January 18, 2021 - 11:34:25 AM
Last modification on : Tuesday, January 19, 2021 - 3:11:27 AM

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INL | CHIMIE | UNIV-MONTPELLIER | UNIV-LYON1 | ICBMS | UNIV-LILLE | INSA-GROUPE | UDL | INRAE | INRA | UGA | CERMAV | EC-LYON | INSA-LYON | CNRS | INC-CNRS

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Caroline Ligeour, Olivier Vidal, Lucie Dupin, Francesca Casoni, Emilie Gillon, et al.. Mannose-centered aromatic galactoclusters inhibit the biofilm formation of Pseudomonas aeruginosa. Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2015, 13 (31), pp.8433-8444. ⟨10.1039/C5OB00948K⟩. ⟨hal-01317684⟩

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