BIOLOGICAL APPLICATIONS OF ATMOSPHERIC PRESSURE DIELECTRIC BARRIER DISCHARGES

Bénédicte Dodet (1) , Emmanuel Odic (1) , Sylvie Salamitou (2) , Alice Goldman (3) , Max Goldman (3)

1 SUPELEC-Campus Gif
2 IGM - Institut de génétique et microbiologie [Orsay]
3 LPGP - Laboratoire de physique des gaz et des plasmas

A reduction of more than 4 orders of magnitude of survivors was obtained by exposing a Bacillus Stearothermophilus spores - contaminated surface to an atmospheric pressure DBD post-discharge for 20 minutes. Decontamination mechanisms are investigated assuming that (i) inactivation is obtained when the bacteria DNA is fragmented, (ii) the protein coats are the main protection of the cell core DNA in the case of bacteria spores. The degradation of DNA (plasmid) and protein (RNAse A) samples submitted to the postdischarge is evaluated according to the operating conditions: gas composition, treatment time and sample state, i.e. hydrated or dried samples.

Keywords: atmospheric pressure non-thermal plasma; suface decontamination; DNA; protein

Domains: Engineering Sciences [physics]; Plasmas

Bénédicte Dodet, Emmanuel Odic, Sylvie Salamitou, Alice Goldman, Max Goldman. BIOLOGICAL APPLICATIONS OF ATMOSPHERIC PRESSURE DIELECTRIC BARRIER DISCHARGES. 10th International Symposium on High Pressure Low Temperature Plasma Chemistry, Sep 2006, Saga, Japan. ⟨hal-00221369⟩