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Potentiel des nanoparticules conductrices à amplifier le champ électrique local pour l’optimisation de l’électroporation et ses applications médicales

Abstract : To improve the outcome of electroporation, a novel approach based on the use of conducive nanoparticles (NPs) is proposed to enhance cell electroperméabilisation. Used as nanoamplifiers, these NPs would provoke a local modification of the electric field around them leading to areas of more intense electric fields. Consequently, the external electric field required to achieve effective cell electropermeabilization could be reduced. During this thesis, the increase of the electropermeabilization effect in the presence of NPs was proved in vitro and in vivo. During the in vitro study, differents compositions and aggregation states of NPs were tested. TEM images were made in order to confirm the location of conductive NPs with respect to the membrane during electroporation. The in vitro study allowed us to find out the appropriate conditions for an optimum interaction of NPs with the cell membrane, leading to a local increase of the electric field. During the in vivo study, the increase of cell electroporation was validated using two types of conductive NPs (spherical and stars). The combination of each type of conductive NPs with electrochemotherapy showed a decrease in the tumor volume with respect to the condition without NPs. These results suggest that the presence of conductive NPs improve the electrochemotherapy outcome via a local amplification of the external electric field. By reducing the applied voltage, the limits of electrochemotherapy will be minimized, which make this antitumor treatment easily applicable.
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https://tel.archives-ouvertes.fr/tel-03282679
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  • HAL Id : tel-03282679, version 1

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Amina Ghorbel. Potentiel des nanoparticules conductrices à amplifier le champ électrique local pour l’optimisation de l’électroporation et ses applications médicales. Cancer. Université Paris-Saclay, 2020. Français. ⟨NNT : 2020UPASQ006⟩. ⟨tel-03282679⟩

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